1201 West Airport Freeway, STE 251
Euless, TX 76040
817-283-2383 - 888-875-7574
Info@Semen-Detection.com

FAQ's | Semen on Panties

FAQ's and Internet Misrepresentation

Prostate Specific Antigen (PSA) Test Information

 

  • The detection of PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations ranging from 2.0x105 to 5.5x106 ng/ml.

 

  • The PSA  test confirms the presence of semen by utilization of a dye-labeled mobile monoclonal antibody that binds to the PSA.

 

  • The PSA antibody complex diffuses horizontally on a membrane with two regions of fixed antibodies. Immobilized polyclonal PSA antibodies are located in a test region and antihuman immunoglobulin antibodies are present in a control region. In these regions, the mobile PSA antibody complex and/or free dye-labeled PSA antibody interact with the immobilized antibody regions.

 

  • Following the application of 200 µl of sample extract and a 10 minute diffusion time, the accumulation of the dye labeled antibodies results in the formation of pink bands at the test and control regions. A pink band at the control region signifies that the test was run appropriately. Immobilization of the antigen antibody complex at both the test and control region positively identifies the presence of the PSA.

Prostate Specific Antigen (PSA) Semen Detection Home Infidelity Test Kit - 

This test is not presumptive like the Acid Phosphatase (AP) test commonly found on the internet.  A positive reaction with a PSA test means the prostate specific antigen found only in semen is present.  

  • PSA Infidelity test kit includes all supplies for one test.

    • PSA test instrument

    • Micro tubes and pipettes for mixing

    • Micro tube with dissolution solution

    • Cutting device with cutting mat

    • Micro tube stand

    • Sterile surgical gloves

    • Instructions

    • Specimen collection pouch with chain of custody documentation.

 

  • How The  PSA Test Is Performed.

    Destructive

    • Cut the article in the area containing the suspected semen samples using the cutting tool.  The suspected substance can also be scraped from the article. 

    • Place in the provided micro-tube and cover the cuttings and/or substance with the dissolution material.

    Non-destructive

    • Swab the stained area with the provided swabs and chemicals and place in the micro-tubes

    Completion of test for destructive or non-destructive method

    • Add provided chemical to the micro-tube and mix.

    • Apply sample to PSA test cartridge.

    • Read results in 10 minutes or less.

 

  • What to do after you receive your results

     

    • Low confidence?  Have this test run by experienced professionals which will also include testing for the amount of semen by Acid Phosphatase (AP), a microscopic inspection and human saliva detection.   We recommend that you do not let the outcome of a home semen detection test result in a life changing situation.

     

    • Positive outcome?  Select this link for one of the follow-on tests which include DNA extraction

     

PSA Misrepresentation on the Internet
  • Here is an example of an online statement made by Evergreen Industries on 30 April 2008.
  • Evergreen's so called recently patented product is an AP test kit patented in July 2004.
  • The PSA cartridge manufacturer's published data is shown below:

     

    Performance Characteristics

    Performance Characteristics The following performance characteristics were observed at a concentration of 2 ng PSA/ml (guaranteed detection limit)

    Diagnostic sensitivity: 100 % 
    Diagnostic specificity: 100 % 
    Positive predictive value: 100 % 
    Negative predictive value: 100 % 
    Reproducibility: 100 %

    and then listed for cancer detection

    For the discrimination of concentration ranges of < and = 4 ng/mL PSA, as it is required for the diagnosis of prostate carcinoma, the performance characteristics of the test were evaluated in two independent clinical trials. The list below shows the mean of the two results:

    Diagnostic sensitivity: 90,0 % 
    Diagnostic specificity: 88,7 % 
    Positive predictive value: 83,3 % 
    Negative predictive value: 96,5 % 
    Reproducibility: 90,2 %

    Notice how Evergreen purposely uses the wrong data to mislead potential consumers about PSA

    ABSOLUTE FACTS ABOUT AP AND PSA TESTS

  • It is an absolute fact that there is no better semen detection test kit available then our PSA kit.  If there is PSA kit equal to ours it's because they copied our design and instructions such as the SemenSpy kit.  We developed the first PSA home kit ever sold so that consumers would quit being duped with the home AP tests.  The PSA cartridge used in our kit is much more sensitive than any other PSA or P30 cartridge tested including the OneStep ABA Card® p30 based on available test data.
  • Evergreen's so called patented product is an AP test kit.
  • It is an absolute fact that all AP tests are presumptive.  Ask any degreed scientist in the industry.  We have 4 PhD's in the industry available for consultation at a nominal charge of $50 (order online).