Frequently Asked Questions About Semen Detections

FAQ on Semen, Sperm, and Vaginal Discharge     FAQ on PSA vs. AP     Infidelity DNA Test

• I think my spouse is cheating on me.  What do I do?

   

  • See this link for signs of infidelity

  • Abstain from having intercourse with him/her for 5-7 days.

  • Order a laboratory semen/sperm/saliva detection and mail in her panties or his underwear to SSDI; 1201 W. Airport Fwy, Ste 251; Euless, TX 76040.

 

• How old of a sample can you test?
  

• The sample can any age.  There is FBI data supporting 30 year old samples testing positive for semen.

• Place any sample in a brown paper bag or envelope.

• Mark the sample envelop with the date the item was worn or the date the sample was obtained if not worn (when possible).

• Positive test results will include this date (when provided) and the results on panties and feminine pads will specify when the intercourse most likely took place. 

 

• I purchased a laboratory test from another company and am not satisfied with the results.

   

  • We will review any semen detection report for free and provide suggestions.  Email your report to Info@Semen-Detection.com or FAX to 817-887-1351.

  • Make sure you do more than just an AP home test which is meaningless alone. 

  • It is also very important not to do DNA testing alone.  You need to have documented proof the the tested item contains semen.

 

• Why are you the only private lab offering saliva detections as part of your semen detection?

 

• Only government crime labs have the capability and experience to perform this test (besides SSDI).  We integrated the infidelity saliva test as part of our semen detection in 2007 

• We have many cases where the suspect is caught by positive saliva results when the semen detection test was negative.  See our testing report section for details on the outcome of most test including negative semen detections with positive human saliva outcomes.

• We are the only private company offering saliva detections to consumers at the time of this update (Jan 2010).  

 

• I ran a home Prostate Specific Antigen (PSA) test and got positive results. 

 

• We recommend that you do not let the outcome of any home semen detection test result in a life changing situation (such as a divorce).  If you confront your spouse before you have 100% conclusive results you will jeopardize your relationship.

• Low confidence on a home test?  Have four different tests run by experienced professionals for only $199.00 that includes the most reliable and sensitive PSA test, an acid phosphatase test which indicates how much of the stain is semen, a microscopic inspection for sperm, and a human saliva detection test.   

• Select this link for one of the follow-on tests which provide DNA profiling.  Please review actual test reports which can assist in your decision.

 

• I ran a home Acid Phosphatase (AP) test (purple or blue dye test) and got positive results. 

   

  • Confirm that it is semen with a home PSA kit or our laboratory test. AP tests are presumptive and a false positive or negative can occur.  Three percent of all AP tests produce a false positive as demonstrated by our statistics.

  • If it is remotely possible that it is your semen, perform a DNA confirmation test. 

  • Home AP tests are often mislabeled as accurate and reliable.  See our frequently asked questions section about the difference between our tests and AP scams.  The reason we classify an Acid Phosphatase (AP) test as a scam is because the significant majority of internet websites do not identify an AP kit as presumptive.  Some websites even classify a home AP kit as 100% accurate.  Every website selling AP kits should tell you that you can receive false positive and negative results.  Here is our home AP kit page as an example.

   

• I ran a home AP test and got negative results. 

   

  • False negatives are common with home AP kits.  AP is detected only up to 72 hours after intercourse.  Thirty-five percent of our lab AP tests produce a false negative as demonstrated by our validation study and statistics.

  • If you have not had intercourse for 7 days or more, you should consider our laboratory test which will provide 100% accurate and conclusive results and is the most sensitive semen detection available.

 

• I was told that a black (UV) light could be use to detect a semen stain.

 

• A black light can be helpful in locating biological stains for testing.  

• No expert can look at a random set of stains and tell you which one is semen.

• We use an expensive selectable multiband UV forensic lamp to fluoresce each article before the extraction is accomplished so that we obtain sampling from every stain.

• The $10 black lights sold on the internet are better than nothing...but not much.

 

• I purchased a SSDI laboratory test with positive results or I am 100% positive I have semen/sperm from another lab or PSA home test.

   

  • How do I identify the male contributor when the sample (such as tissue or garment) I have consists of semen/sperm only and was not contaminated by the female presence (such as vaginal discharge from panties or a feminine pad)?

    • You would order a PowerPlex DNA extraction from semen/sperm or the complete PowerPlex sperm determination.

  • How do I identify the male contributor when the sample (such as panties or feminine pad) consists of semen/sperm and probable contamination from a female but I am only trying to identify the male contributor?

    • You would order a YPLEX DNA extraction from semen/sperm or the complete YPLEX sperm determination.

  • How do I identify the male and female contributor when the male contributor is not vasectomized or I am not sure if he has a vasectomy (panties, feminine pad, oral sex stain, or a condom)?

    • You would order a differential lysis which will provide a separate PowerPlex profile on the male and female if the male is present by sperm cells and the female is present by epithelia  or buccal cells.  A differential lysis consists of 2 separate tests.

      • It is possible that there is heavy contamination by the male presence (such as buccal cells from oral sex) and the extractions produce a combined profile consisting of more than one contributor.

      • It is possible that the sperm cells have lysed (dissolution or destruction) and both the male and female fractions of the extraction may be similar or identical.

        • Even when the profiles are mixed the results can be helpful in proving that a specific contributor is included or excluded from the stain.

  • How do I identify the male and female contributor when the male contributor is vasectomized  (panties, feminine pad, oral sex stain, or a condom)?

    • You would order a PowerPlex profile from a semen stain.  You will most likely obtain a mixed profile of more than one contributor.

    • Even when the profiles are mixed the results can be helpful in proving that a specific contributor is included or excluded from the stain.

  • Why didn't my DNA extraction produce a male profile when I was 100% positive that semen was present?

    • DNA is found in sperm, not semen. If an individual is vasectomized or azoospermic (producing no sperm), DNA will only be available from the epithelia cells shed during ejaculation. The success rate of DNA extraction of a vasectomized or azoospermic individual is greatly reduced but still possible.  The most sensitive extraction and profiling is by YPLEX.

    • The sample was badly degraded which is normally caused by bacteria attacking the cells which inhibit the binding of the primers during the PCR amplification process.  Storing a moist sample in a Ziploc bag is the number one cause of this type of failure.  If you see mold or mildew on a sample you should expect it not to work.

    • It is possible that the adulterer is vasectomized and you may want to consider other methods of catching the cheaters by hiring an infidelity Private Investigator.

  • What will my test results look like?

    • Please see the actual test examples section of our website.

  • How do I order?

    • Order online or by phone or by chat .

 

• What if the questions I have sound stupid or I'm not sure what to ask.

  • There is no such thing as a stupid question when you are needing assistance.  We are here to get the answers you need to move forward with your life.

  • We do receive questions that are funny but you will receive a respectful reply.

Prostate Specific Antigen (PSA) Semen Detection Home Infidelity Test Kit - 

Order a home PSA test kit

This test is not presumptive like the Acid Phosphatase (AP) test commonly found on the internet.  A positive reaction with our PSA test means the prostate specific antigen found only in semen is present.  

• PSA Infidelity test kit includes all supplies for one test.

  • PSA test instrument

  • Micro tubes and pipettes for mixing

  • Micro tube with dissolution solution

  • Cutting device with cutting mat

  • Micro tube stand

  • Sterile surgical gloves

  • Instructions

  • Specimen collection pouch with chain of custody documentation.

• How Our  Home PSA Test Is Performed.

  Destructive

  • Cut the article in the area containing the suspected semen samples using the cutting tool.  The suspected substance can also be scraped from the article. 

  • Place in the provided micro-tube and cover the cuttings and/or substance with the dissolution material.

  Non-destructive

  • Swab the stained area with the provided swabs and chemicals and place in the micro-tubes

  Completion of test for destructive or non-destructive method

  • Add provided chemical to the micro-tube and mix.

  • Apply sample to PSA test cartridge.

  • Read results in 10 minutes or less.

SSDI is a Texas Corporation owned and operated by DNA Testing Centre.