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Prostate Specific Antigen (PSA) Test
Information
- The detection of PSA on forensic samples is often helpful
because it confirms the presence of semen even in samples that
involve vasectomized or azoospermic individuals. PSA is a
glycoprotein produced by the prostate gland and secreted in
seminal fluid at concentrations ranging from 2.0x105 to
5.5x106 ng/ml.
- The PSA test confirms the presence of semen by
utilization of a dye-labeled mobile monoclonal antibody that
binds to the PSA.
- The PSA antibody complex diffuses horizontally on a membrane
with two regions of fixed antibodies. Immobilized polyclonal
PSA antibodies are located in a test region and antihuman
immunoglobulin antibodies are present in a control region. In
these regions, the mobile PSA antibody complex and/or free
dye-labeled PSA antibody interact with the immobilized
antibody regions.
- Following the application of 200 µl of sample extract and a
10 minute diffusion time, the accumulation of the dye labeled
antibodies results in the formation of pink bands at the test
and control regions. A pink band at the control region
signifies that the test was run appropriately. Immobilization
of the antigen antibody complex at both the test and control
region positively identifies the presence of the PSA.
Prostate Specific
Antigen (PSA) Semen Detection Home Infidelity Test Kit -
This test is not presumptive like the Acid Phosphatase (AP)
test commonly found on the internet. A
positive reaction with a PSA test means the prostate specific
antigen found only in semen is present.
PSA Misrepresentation
on the Internet
- Here is an example of an online
statement made by Evergreen Industries on 30 April 2008.
- Evergreen's so called recently patented product is an AP
test kit patented in July 2004.
- The PSA cartridge manufacturer's published data is shown
below:
Performance Characteristics
Performance
Characteristics The following performance characteristics
were observed at a concentration of 2 ng PSA/ml
(guaranteed detection limit)
Diagnostic
sensitivity: 100 %
Diagnostic specificity: 100 %
Positive predictive value: 100 %
Negative predictive value: 100 %
Reproducibility: 100 %
and
then listed for cancer detection
For
the discrimination of concentration ranges of <
and = 4 ng/mL PSA, as it is required for the
diagnosis of prostate carcinoma,
the performance characteristics of the test were evaluated
in two independent clinical trials. The list below shows
the mean of the two results:
Diagnostic
sensitivity: 90,0 %
Diagnostic specificity: 88,7 %
Positive predictive value: 83,3 %
Negative predictive value: 96,5 %
Reproducibility: 90,2 %
Notice how Evergreen purposely
uses the wrong data to mislead potential consumers
about PSA
ABSOLUTE FACTS
ABOUT AP AND PSA TESTS
- It is an absolute fact that there is no better semen
detection test kit available then our PSA kit. If
there is PSA kit equal to ours it's because they copied our
design and instructions such as the SemenSpy kit. We
developed the first PSA home kit ever sold so that consumers
would quit being duped with the home AP tests. The PSA
cartridge used in our kit is much more sensitive than any
other PSA or P30 cartridge tested including the OneStep ABA
Card® p30 based on available test
data.
- Evergreen's so called patented product is an AP
test kit.
- It is an absolute fact that all AP tests are presumptive.
Ask any degreed scientist in the
industry. We have 4 PhD's in the industry available for consultation
at a nominal charge of $50 (order
online).
- False positive results will happen because Acid
phosphatase is an enzyme present in the cells throughout
the body. It is present in especially high concentrations
in the prostate and semen in men. Many other
substances contain high AP.
- False negative results will happen because AP cannot be
detected in samples containing less then 78 ng and demonstrated
by our validation study
and other white
papers.
- See the last paragraph of the Virginia State Crime Lab
Manual where the results of a positive AP test alone are
identified as inconclusive http://www.semenonpanties.com/VirginiaForensicScience.pdf
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