Semen and Sperm Detection test report on a semen detection with negative Prostate Specific Antigen (PSA), positive Acid Phosphatase (AP) and negative for sperm identification.

Other Tests     Semen Detection Statistics

The customer (husband) had purchased a home semen detection test kit and received positive results.  Upon discussing additional testing options with one of our representatives he decided to order a professional laboratory semen detection in lieu of a DNA extraction.  The results of this test contradicted the home test results.

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(Report is identical to released notarized report except customer information was removed)

Analysis Report

Case #  PSA51531A-C

Evidence

Item A is one pair of ladies panties, Victoria Secret, color white w/purple, size M, R/N 54867.  Item B is one pair of ladies panties, Victoria Secret, color pink w/white, size M, R/N 54867.  Item C is one pair of ladies panties, Victoria Secret, color gray w/white, size M, R/N 54867.  UV long and short wave light confirmed the locations for testing.  

Analysis

Tests for the presence of Acid Phosphatase (AP) was conducted.  Acid phosphatase is an enzyme present in semen at concentrations of 20 to 400 times other body fluids.  The presence of acid phosphatase is a presumptive test for the presence of semen and needs to be confirmed by DNA or the presence of a Prostate Specific Antigen (PSA).  

A Prostate Specific Antigen (PSA) test was completed on each item.  The detection of the PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. The PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml). 

A microscopic examination was completed on each item.  Microscopic examination is a presumptive test for the presence of sperm unless intact, non-degraded sperm are detected.  Intact sperm are rarely visible after 4 days unless the sample was immediately frozen and properly stored.

Results

All tests were negative for the presence of semen.  No intact sperm heads were detected microscopically, and the PSA and AP tests were negative.

Conclusions

All three samples tested negative for the presence of semen.  The samples were negative for PSA and AP and for the visual microscopic analysis for sperm and PSA.  The samples and proof of tests will be held for 90 days then destroyed unless requested by customer.