False Positive Semen Detection Test Results By Acid Phosphatase!

Semen and Sperm Detection test report on a semen detection with negative Prostate Specific Antigen (PSA), positive Acid Phosphatase (AP) and negative for sperm identification.

Other Tests     Semen Detection Statistics

The customer (husband) suspected his wife of having an affair.  The test Acid Phosphatase (AP) portion of his test was positive but the PSA portion of his test was negative so the semen detection was repeated and confirmed that his test was negative for semen.  A home or laboratory test for Acid Phosphatase (AP) alone would have provided the wrong result.

Click on picture for full size

(Report is identical to released notarized report except customer information was removed)

Analysis Report

Case #  PSA71838

Evidence

The item to be tested is one pair of pumpkin (dark orange) ladies panties with a worn ID tag where the manufacture or size could not be identified.  UV long and short wave light confirmed the locations for testing.  Photographs were taken.

Analysis 

A test for the presence of Acid Phosphatase (AP) was conducted.  Acid phosphatase is an enzyme present in semen at concentrations of 20 to 400 times other body fluids.  The presence of acid phosphatase is a presumptive test for the presence of semen and needs to be confirmed by DNA or the presence of a Prostate Specific Antigen (PSA).  

A Prostate Specific Antigen (PSA) test was completed.  The detection of the PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. The PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml).

A microscopic examination was completed.  Microscopic examination is a presumptive test for the presence of sperm unless intact, non-degraded sperm are detected.  Intact sperm are rarely visible after 4 days unless the sample was immediately frozen and properly stored.

A test for the presence of human salivary amylase, an enzyme found in human saliva was performed.  This test will detect as little as 1 µl of human with no cross-reaction to blood, semen, urine, vaginal secretions or menstrual blood.

Results

The AP test was positive with a moderate rating of 6. All confirmation tests were negative for the presence of semen.  PSA was not detected and no intact sperm heads were detected microscopically. A RSID saliva test was performed and was also negative.  An additional PSA test was performed on the AP sample that was also negative.

Conclusions

The test was negative for the presence of semen by PSA and microscopic examination and negative for the presence of saliva.  DNA extraction for a male profile is not recommended.  The proof of test will be held in freezer for 90 days then destroyed unless requested by the customer.  The tested sample and results were mailed to the address below.