Semen and Sperm Detection test report on a semen detection with positive Prostate Specific Antigen (PSA), negative Acid Phosphatase (AP) and negative for sperm identification.

Other Tests     Semen Detection Statistics

The customer (husband) suspected that his wife had intercourse the previous night with her boss.  The semen detection was positive but the results indicated that intercourse took place 4-6 days before the panties were worn.  The customer could not remember when he last had intercourse with his wife so he ordered the DNA extraction to be safe.  The DNA extraction from the panties match his DNA.

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(Report is identical to released notarized report except customer information was removed)

Analysis Report

Case #  PSA71889

Evidence

The item to be tested is one pair of white Victoria’s Secret Hi-Leg Briefs size XL.  UV long and short wave light confirmed the locations for testing.  

 Analysis 

A test for the presence of Acid Phosphatase (AP) was conducted.  Acid phosphatase is an enzyme present in semen at concentrations of 20 to 400 times other body fluids.  The presence of acid phosphatase is a presumptive test for the presence of semen and needs to be confirmed by DNA or the presence of a Prostate Specific Antigen (PSA).

A Prostate Specific Antigen (PSA) test was completed.  The detection of the PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. The PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml).

A microscopic examination was completed.  Microscopic examination is a presumptive test for the presence of sperm unless intact, non-degraded sperm are detected.  Intact sperm are rarely visible after 4 days unless the sample was immediately frozen and properly stored.

A test for the presence of human salivary amylase, (HSA) an enzyme found in human saliva was performed.  This test will detect as little as 1 µl of human saliva with no cross-reaction to blood, semen, urine, vaginal secretions or menstrual blood.

Results

The PSA test was positive.  No AP was detected.  No whole sperm heads were detected microscopically which may be attributed to the age of the sample or the contributor could be vasectomized or azoospermic.  A RSID saliva test (HSA) was performed which was negative for human saliva.

Conclusions

The item tested positive for semen and negative for saliva.  The stained area tested PSA positive for semen and negative for AP and the visual microscopic analysis for sperm.  The results imply intercourse occurred between 3 and 5 days of when the panties were last worn.  A successful DNA extraction for a male YPLEX profile is estimated above 65%.  The tested item and proof of test will be held in freezer for one year and then destroyed unless requested by customer.  The results will be sent to the address below.