Semen and Sperm Detection test report on a semen detection with positive Prostate Specific Antigen (PSA), negative Acid Phosphatase (AP) and negative for sperm identification.

Other Tests     Semen Detection Statistics

The customer (husband) suspected that his wife had intercourse the previous night with her boss.  The semen detection was positive but the results indicated that intercourse took place 4-6 days before the panties were worn.  The customer could not remember when he last had intercourse with his wife so he ordered the DNA extraction to be safe.  The DNA extraction from the panties match his DNA.

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(Report is identical to released notarized report except customer information was removed)

Analysis Report

Case #  PSA71823

Evidence

The item to be tested is one pair of ladies panties manufactured by Q-T, color Pink, size 5, R/N 16236.   UV long and short wave light confirmed the locations for testing.  

Analysis 

A test for the presence of Acid Phosphatase (AP) was conducted.  Acid phosphatase is an enzyme present in semen at concentrations of 20 to 400 times other body fluids.  The presence of acid phosphatase is a presumptive test for the presence of semen and needs to be confirmed by DNA or the presence of a Prostate Specific Antigen (PSA).  

A Prostate Specific Antigen (PSA) test was completed.  The detection of the PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. The PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml).

A microscopic examination was completed.  Microscopic examination is a presumptive test for the presence of sperm unless intact, non-degraded sperm are detected.  Intact sperm are rarely visible after 4 days unless the sample was immediately frozen and properly stored.

Results

The PSA test was positive.  The acid phosphatase was negative.  No whole sperm heads were detected microscopically which may be attributed to the age/size/condition of the sample or the contributor could be vasectomized or azoospermic.  A RSID saliva (new trial) test was performed and was negative.

Conclusions

The item tested positive for semen.  The stained area tested PSA positive for semen and negative for the visual microscopic analysis for sperm.  The AP portion of the test was negative indicating that the visible stain was not semen.  The PSA outcome indicates that intercourse took place 4-6 days prior to the panties being worn.  Potential successful DNA extraction from the sample is estimated to be above 30%.  The sample and proof of test will be held in freezer for one year then destroyed unless requested from customer.  Results are emailed at this time.

DNA Extraction from the tested article

Case # FR2007771 (GQ0018344) 

Evidence 

The item to be tested is R1- cloth punches from panties and R2- 2 buccal swabs labeled “Customer”. 

Analysis 

Cell lysis, DNA extraction, PCR and Genotyper was performed on a small section of the sample. In addition, a YPLEX PCR analyzing the alleles DYS389I, DYS439, DYS438, DYS437, DYS392, DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 was also performed on the samples. 

Results:
Y-PLEX ANALYSIS RESULTS: Allele and Number of Repeats

Conclusions:

Based on the analysis of the alleles DYS389I, DYS439, DYS438, DYS437, DYS392, DYS393, DYS19, DYS389II,
DYS390, DYS391, and DYS385, the profile obtained from Sample R1 and R2 are identical profiles consistent with
male DNA. This profile occurs in approximately 2 of 4004 male members of the General population.