Semen and Sperm Detection test report on a semen detection with positive Prostate Specific Antigen (PSA), Acid Phosphatase (AP) and Negative for sperm identification.

Other Tests     Semen Detection Statistics

These five samples were completed on one report (from the same customer/time).

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(Report is identical to released notarized report except customer information was removed)

Analysis Report

Case #  PSA71858A-E

Evidence

Item A was manufactured by Victoria’s Secret, color white, size M, RN41321.  Item B was manufactured by Victoria’s Secret, trade name “very sexy”, color white, size M, RN41402.  Item C was manufactured by Victoria’s Secret, trade name “sexy little things”, color black with pink polka dots, size M, RN70817.  Item D was manufactured by Victoria’s Secret, trade name “sexy little things”, color black, size M, RN72409.  Item E was manufactured by Venus, color black, size 6, RN78426.

Analysis

A test for the presence of Acid Phosphatase (AP) was conducted.  Acid phosphatase is an enzyme present in semen at concentrations of 20 to 400 times other body fluids.  The presence of acid phosphatase is a presumptive test for the presence of semen and needs to be confirmed by DNA or the presence of a Prostate Specific Antigen (PSA).  

A Prostate Specific Antigen (PSA) test was completed.  The detection of the PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. The PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml).

A microscopic examination was completed.  Microscopic examination is a presumptive test for the presence of sperm unless intact, non-degraded sperm are detected.  Intact sperm are rarely visible after 4 days unless the sample was immediately frozen and properly stored.

A test for the presence of human salivary amylase (HSA), an enzyme found in human saliva was performed.  This test will detect as little as 1 µl of human saliva with no cross-reaction to blood, semen, urine, vaginal secretions or menstrual blood.

Results

Item A tested negative for the presence of semen by AP, PSA and microscopic examination.  Items B, C, D, and E tested positive for semen.  Items A, D, and E tested negative for human saliva.  Items B and C tested positive for human saliva.  The following chart provides the comparative strengths of the results of each sample and the four tests (1 to 10 scale, 10 being the strongest outcome).

Conclusions

Items A tested negative for the presence of semen by AP, PSA and human saliva.  Items B thru E tested positive for semen.  Items B and C tested positive for human saliva.  No whole sperm heads were detected microscopically on any item which may be attributed to the age or amount of the sample or the contributor could be vasectomized or azoospermic.  Successful DNA extraction for a male profile is estimated to be above 80% on item C and D and above 70% on Items B and E.  Intercourse most likely occurred within 8 hours on item C, within 12 hours on items B and D, and within 24 hours on item E.