Semen and Sperm Detection test report on a semen detection with positive Prostate Specific Antigen (PSA), Acid Phosphatase (AP) and Negative for sperm identification.

Other Tests     Semen Detection Statistics

Once the customer received the positive results of semen detection, he ordered a YPLEX DNA extraction from the panties which yielded a clear profile on one male.  The customer had observed his wife flirting a few times with the same individual so he followed him and retrieved his discarded Gatorade bottle. He not only caught his wife cheating but also proved whom she was cheating with.  See DNA report below.

Panties

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(Report is identical to released notarized report except customer information was removed)

Analysis Report

Case #  PSA71863

Evidence

The item to be tested is one pair of dark blue Victoria’s Secret ladies panties with a worn imprinted label.  UV long and short wave light confirmed the locations for testing.  Several hairs and fibers were collected from the sample and stored with the proof of test.

Analysis 

A test for the presence of Acid Phosphatase (AP) was conducted.  Acid phosphatase is an enzyme present in semen at concentrations of 20 to 400 times other body fluids.  The presence of acid phosphatase is a presumptive test for the presence of semen and needs to be confirmed by DNA or the presence of a Prostate Specific Antigen (PSA).  

A Prostate Specific Antigen (PSA) test was completed.  The detection of the PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. The PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml).

A microscopic examination was completed.  Microscopic examination is a presumptive test for the presence of sperm unless intact, non-degraded sperm are detected.  Intact sperm are rarely visible after 4 days unless the sample was immediately frozen and properly stored.

A test for the presence of human salivary amylase, (HSA) an enzyme found in human saliva was performed.  This test will detect as little as 1 µl of human saliva with no cross-reaction to blood, semen, urine, vaginal secretions or menstrual blood.

Results

The AP and PSA test were strong positive.  No whole sperm heads were detected microscopically which may be attributed to the age of the sample or the contributor could be vasectomized or azoospermic.  A RSID saliva test (HSA) was performed which was positive for human saliva.

Conclusions

The item tested positive for semen and saliva.  The stained area tested AP and PSA positive for semen and negative for the visual microscopic analysis for sperm.  The results imply intercourse occurred within 12 hours of when the panties were last worn.  A successful DNA extraction for a male YPLEX profile is estimated above 85%.  The tested item and proof of test will be held in freezer 1 for one year and then destroyed unless requested by customer.  The results will be sent to the address below.

Select graphic for actual tested bottle

DNA Evidence 

The item to be tested is Sample R1- 1.7 ml tube labeled “cloth punches in PBS” and Sample R2- 1.7ml tube containing 2 swab tips labeled “Gatorade bottle”. 

Analysis 

Cell lysis, DNA extraction, PCR and Genotyper was performed on a small section of the sample. In addition, a Y-PLEX PCR analyzing the alleles DYS389I, DYS439, DYS438, DYS437, DYS392, DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 was also performed on the sample. 

Results:
Y-PLEX ANALYSIS RESULTS: Allele and Number of Repeats

Conclusions: