Semen and Sperm Detection test report on a semen detection with positive Prostate Specific Antigen (PSA), Acid Phosphatase (AP) and positive for sperm identification.

Other Tests     Semen Detection Statistics

The customer's wife went to Wal-Mart at 3:00 am and returned with stains on her dress.  

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(Report is identical to released notarized report except customer information was removed)

Analysis Report

Case #  PSA71934

Evidence

The item to be tested is pink cloth cutting 9.5x7 inches.  UV long and short wave light was confirmed the locations for testing. 

Analysis 

A test for the presence of Acid Phosphatase (AP) was conducted.  Acid phosphatase is an enzyme present in semen at concentrations of 20 to 400 times other body fluids.  The presence of acid phosphatase is a presumptive test for the presence of semen and needs to be confirmed by DNA or the presence of a Prostate Specific Antigen (PSA).  

A Prostate Specific Antigen (PSA) test was completed.  The detection of the PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. The PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml).

A microscopic examination was performed.  Microscopic examination is a presumptive test for the presence of sperm unless intact, non-degraded sperm are detected.  Intact sperm are rarely visible after 4 days unless the sample was immediately frozen and properly stored.

A test for the presence of human salivary amylase, (HSA) an enzyme found in human saliva was performed.  This test will detect as little as 1 µl of human saliva with no cross-reaction to blood, semen, urine, vaginal secretions or menstrual blood.

 Results

The AP and PSA test were positive and whole sperm heads were detected microscopically.  The RSID saliva test (HSA) was also positive for human saliva.

 Conclusions

The rating scale is from 1 to 10, ten being the strongest positive outcome.  The item tested positive for semen and for human saliva (9).  The stained area tested AP (4) and PSA (10) positive for semen and positive for the visual microscopic analysis for sperm (10).  The results imply that the item contains semen and sperm but a significant amount of the stain was not semen.  A successful DNA extraction for a male YPLEX profile is estimated above 95%.  The tested item and proof of test will be held in freezer for one year and then destroyed unless requested by customer.  The results will be mailed to the address below.