Semen and Sperm Detection test report on a semen detection with positive Prostate Specific Antigen (PSA), Acid Phosphatase (AP) and positive for sperm identification.

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(Report is identical to released notarized report except customer information was removed)

Analysis Report

Case #  PSA71855

Evidence

The item to be tested is off-white cloth bag with no manufacturers tag, size 17x7.5 inches.  UV long and short wave light confirmed the locations for testing.  The fluoresced areas were marked and 2 photographs were taken.

Analysis 

A test for the presence of Acid Phosphatase (AP) was conducted.  Acid phosphatase is an enzyme present in semen at concentrations of 20 to 400 times other body fluids.  The presence of acid phosphatase is a presumptive test for the presence of semen and needs to be confirmed by DNA or the presence of a Prostate Specific Antigen (PSA).  

A Prostate Specific Antigen (PSA) test was completed.  The detection of the PSA on forensic samples is often helpful because it confirms the presence of semen even in samples that involve vasectomized or azoospermic individuals. The PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml).

A microscopic examination was completed.  Microscopic examination is a presumptive test for the presence of sperm unless intact, non-degraded sperm are detected.  Intact sperm are rarely visible after 4 days unless the sample was immediately frozen and properly stored.

A test for the presence of human salivary amylase, (HSA) an enzyme found in human saliva was performed.  This test will detect as little as 1 µl of human with no cross-reaction to blood, semen, urine, vaginal secretions or menstrual blood.

Results

The AP and PSA test was strong positive.  Whole sperm heads were detected microscopically.  A RSID saliva test (HSA) was performed which was negative for human saliva.

Conclusions

The item tested strong positive for semen and tested negative for saliva.  The stained area tested AP and PSA positive for semen and positive for the visual microscopic analysis for sperm.  A successful DNA extraction for a male profile is estimated above 95%.  The tested item and proof of test will be held in freezer 1 for one year and then destroyed unless requested by customer.  The results will be sent to the address below.

DNA Extraction from the tested article

Evidence

The item to be tested is: R1- a semen stain in a cloth bag for PowerPlex.   

Analysis 

DNA extraction, PCR and Genotyper were performed on the sample R1 and the DNA profile is below.   

DNA ANALYSIS RESULTS: Allele and number of repeats 

Conclusions: