A case of a differential lysis for female infidelity

The customer ordered online and when contacted to discuss his situation his disclosure was limited due to embarrassment.   The customer would not disclose if he had a vasectomy or how old the sample was or how it was stored.  No formal semen detection was performed on the sample because the customer was adamant that semen was present.  The YPLEX test data was released and the customer was contacted to discuss the results.  At this time it was disclosed that it was possible that his brother was cheating with his wife.  Since paternal brothers share the same Y chromosomes, the customer elected to order a differential lyses.  The results of the differential lysis clearly demonstrate that the customer (husband)  is the contributor to the stain. 

Analysis Report 

Case # FR2007838 (GQ0018636) 

On October 03, 2007 an envelope was delivered by Federal Express (Tracking Number 7924 2992 XXXX.  The contents sample R1 and R2 were checked in and stored until analysis. On October 10, 2007 an envelope was delivered by Federal Express (7914 0697 XXXX). The content sample R3 was checked in and stored until analysis. 

Evidence 

The items to be tested are: R1- one swab packet labeled “Customer” dated 09/29/07 1:00 PM for PowerPlex; R2-one 1.7 mL tube labeled “YPLEX for Panties”; R3- 1.7 ml tube labeled “panties” for differential lysis. 

Analysis 

DNA extraction, PCR and Genotyper were performed on the samples R1 and R2. In addition, a Y-PLEX PCR analyzing the alleles DYS389I, DYS439, DYS438, DYS437, DYS392, DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 was also performed on the samples R1 and R2. A differential lysis was performed on sample R3 to separate sperm and epithelial cells in different groups allowing a profile for any contributors to the stain to be profiled, followed by DNA extraction, PCR and Genotyper 

Results: 

Y-PLEX ANALYSIS RESULTS: Allele and Number of Repeats 

* Minor peak. 

Conclusions: 

Based on the analysis of the alleles DYS389I, DYS439, DYS438, DYS437, DYS392, DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385, the profiles obtained from Sample R1 and Sample R2 are consistent with male DNA. The two profiles obtained for Sample R1 and Sample R2 are identical. This profile occurs in approximately greater than 1 of 894 male members of the Hispanic population.  Based on the analysis of the alleles systems D3S1358, THO1, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA and Amelogenin from the differential lysis Sample R1, "Customer", cannot be excluded as a contributor as Sample R3.