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DNA extraction from used condom for female DNA.

The husband found the use condom on the floor in the living room.  The wife declared it was his or someone else.  The condom extraction matched the cigarette butt control sample from the wife so she was caught.  It is important to handle the condom properly to ensure this type of test is performed.  Our extraction and sample handling instructions and methods will insure the best test possible.

 

Analysis Report

Case # FR2004456  (GQ0016929)

 Evidence

 

The first item to be tested is R1- two swabs in a 1.7ml tube labeled as “GQ0016929 condom”.  The second item is R2- two separate 1.7ml tubes containing small pieces of paper, labeled as “GQ0016929 butt”. 

 

Analysis 

DNA extraction, PCR and Genotyper were performed on the two samples and the DNA profiles are compared below. 

 

DNA ANALYSIS RESULTS: Allele and number of repeats

 

Alleles

R1: Condom

R2: Cigarette Butts

CSF1P0

10, 12

10, 12

D2S1338

18, 19

18, 19

D3S1358

15

15

D5S818

10, 13

10, 13

D7S820

10

10

D8S1179

13

13

D13S317

11, 12

11, 12

D16S539

9, 13

9, 13

D18S51

17, 18

17, 18

D19S433

13, 15

13, 15

D21S11

32.2

32.2

FGA

20, 22

20, 22

THO1

7, 9.3

7, 9.3

TPOX

8, 11

8, 11

vWA

16, 17

16, 17

Amelogenin

X

X

 

Conclusions:

 

The profiles obtained from R1-the two swabs labeled as “GQ0016929 condom” in the 1.7ml tube and R2- the small pieces of paper labeled as “GQ0016929 butt” in the 1.7ml tube are identical.