110 East Broward Blvd. Suite 1700
Fort Lauderdale, Florida 33301
517-336-7734 or 888-875-7574


For Infidelity and Sexual Assault

Semen and Sperm Detection, Inc. provides 100% accurate and conclusive analysis for the detection of semen, sperm, and human saliva (and blood if applicable) to determine whether infidelity or rape has occurred. Our 4-phase testing process is the most accurate and reliable semen and saliva detection test available. A comprehensive written report is provided for each case, including photographic documentation. Tests are typically completed within one business day from receipt of the sample(s).


Initiate Mobile Device Chat

Semen Detections are primarily utilized in determining infidelity or rape.   

Semen on Panties

Panties (examples) are tested more than all other items combined.  Male underwear is the second most tested item.  Common items include pants, feminine pads and tampons, bed linens, scrapings, washcloths, bathrobes, t-shirts, pants, pizza, blouses, lingerie, soft drinks, and juices, to name a few.

Do not terminate your relationship due to results of a home semen detection test kit. example1  example2 example3 

If you use a home AP test there is a 35% chance that you will receive a false negative.


Sexual Assault Case Relief


What Differentiates Us

Semen and Sperm Detection, Inc. has performed over 3200 semen detections and has sold over 50,000
Prostate Specific Antigen (PSA) kits.

SSDI performs the most accurate and definitive semen detection test available.

Positive semen detection reports identify to extreme specifics the strength of each outcome.  When panties or feminine pads are tested the results provide the time frame the intercourse took place.  This level of detail and expertise is unparalleled.   

Sexual assault assistance

Actual test reports

Recent news, contracts, and awards

Validation studies

Semen detection statistics

History of semen detections

Infidelity Private Investigator

Frequently Asked Questions (FAQ)

Home infidelity test kits

Active customer feedback


::DNA Extraction From Semen/Sperm
In the event your sample is positive for semen or sperm, you may elect to proceed with DNA analysis for further confirmation.  The most common DNA extractions are the Y-PLEX, specific for male DNA, or the PowerPlex 18 Profile. Each of these extraction types are highly successful of producing a DNA profile . This evidence may then be used for comparison testing to determine if an individual is likely to be a contributor or is excluded as a contributor to the sample. Please see this link for additional types of follow up DNA tests that may be accomplished on a semen or sperm sample.


Expert Witness
Testimony - Criminal Case

I hired SSDI on behalf of a client who had been charged with indecency with a Child. SSDI went to the alleged scene of the crime to look for any physical evidence that would support the allegations against my client. From the initial sweep with an ultra-violet light to the comprehensive semen detection testing, SSDI did all the things the detectives on the case should have done for the prosecution. SSDI provided an excellent report regarding their procedures and their findings. Ultimately, the report served as the basis of SSDI's testimony at my client's jury trial. He was easily qualified before the Court as an expert and neither his credentials nor his report was seriously challenged. My client was acquitted after a brief jury deliberation. The jurors discussed the case with me after the verdict. The jurors agreed that they were very impressed with the work SSDI did on the case. They cited the work and testimony of SSDI as one of the primary reasons they returned a verdict of "Not Guilty." My client and I could not be happier with SSDI. I plan to use them again in cases like this one because they give me and my clients the advantage we need for trial.

Attorney D.P. from Denton Texas


::Civil Case

The case settled without going to court. Final papers will be signed March 5th. She said to tell you thanks for what you did and the testing was a major factor in the settlement.

JT - Dallas

Customer Service Hours in Eastern Standard Time

Monday - Friday 9:00 AM to 5:00 PM EST



Negative Semen
Customer Testimonial

I never expected that I would catch my girlfriend cheating by having oral sex performed on her by her friend. I sent SSDI two pair of my girlfriend’s panties retrieved from a hotel room. The next day I received a detailed test report demonstrating that both pair of panties were negative for semen but positive for saliva. I needed closure to this so I ordered DNA profiling. A male profile was obtained and compared to my mouth swab and the two profiles were different. I confronted my girlfriend and she called me crazy and agreed to get a sample from the person I suspected (her friend) and also take a lie detector test. SSDI advised me to call her bluff and after attempting to get the suspects sample my girlfriend moved out. SSDI provided excellent assistance before, during, and after the testing.  Scott from Ohio





Semen, Sperm, and Saliva Detection

We resolve 91.9% of all infidelity issues within 24 hours of sample receipt (Monday-Friday - see statistics)

Questions?     Order     Mobile     Contact Us

Confirm infidelity, rape, early teen sexual activity, 
and child molestation.

With over 3200 completed tests, SSDI promises the most accurate, sensitive, and comprehensive laboratory test available, within one business day of sample receipt!

Active Customer Feedback

  1. Order serology test by credit card, PayPal, or money order

  2. Please ship the garment or item to be tested in a paper bag or envelope. On the outside of the paper bag or envelope, write your contact information and date you obtained the sample. Please DO NOT use a mailer or poly / plastic bag or wrapping around the garment, as this may cause the item to sweat and destroy the sample.

  3. Send sample to SSDI, 110 East Broward Blvd. Suite 1700 Fort Lauderdale, Florida 33301 in envelope or paper bag

     Statistics     Test Reports     FAQ     DNA

$245 Lab Serology Detections Include

Unparalleled Report Example

  • Prostate specific antigen for semen
  • Microscopic sperm assessment
  • Acid Phosphatase (AP) evaluation
  • Human saliva for oral sex
  • Human blood when warranted
  • Sperm Protein (SP10) when warranted
  • Reports can be mailed, emailed, faxed, or provided by phone.
    • Report contains photo of tested item(s)
    • Report specifies the time frame of intercourse when panties or feminine pads test positive
    • Report provides relative chance of obtaining successful DNA profile on positive samples
  • Free sample storage after test is completed

Highly recommended by DNA Laboratories, Attorneys,
Private Investigators, Forensic Specialists, and private individuals.  See our active customer feedback.


Are you an attorney needing criminal case support, confirmation testing, or serology and DNA documents reviewed?

Customer testimonial from a legal sexual assault case

SSDI is top notch! My test results were available to me in less than 6 hours from when the sample was received by SSDI and I didn't even pay extra for expedited service! All of my questions were clearly explained to me and your staff were very sensitive as to the nature of my questions. I would definitely recommend your service to anyone! Very professional, very quick, very thorough... everything one would want in such a service! See Survey


100% Confidential 

Services and Fees

Expert witness testimony support for sexual assault and infidelity

517-336-7734 OR
Toll Free 888-875-7574

Zero Customer Complaints

We have never received a customer complaint regarding a lab serology test...ever!


AABB and ISO17025 Accredited Lab Testimonial

My laboratory has had the pleasure of working with the representatives of SSDI for over 8 years. I personally trained Steve Calvert at our lab in 2002 during the time period when we offered semen detections. Their representatives are well trained, experienced, knowledgeable, and professional. The significant majority of their positive semen detections processed for DNA yield a useful profile. I have full confidence in SSDI's semen detection testing and DNA preparation. Our lab often refers semen detection customers and dealers to SSDI.



Customer Testimonial

Thanks so much for solving this for me. Not only was the speed very impressive but the thoroughness of your analysis was just what I needed to force my wife to tell me the truth after 2 years of deceit and lies. This was just beautiful!!!
 Sincerely, Steven from Florida
(read more)


Testing Performed With Each Order

  • Prostate Specific Antigen (PSA) .  PSA is produced by the prostate gland (male specific) and secreted in seminal fluid. Unlike the acid phosphatase presumptive test, detection of PSA requires the presence of the semen protein without the need of the target to perform an enzymatic function.   (additional PSA information).
  • Microscopic inspection - The substance is wet mounted and examined under a high power phase microscope for the presence of sperm.
  • Acid Phosphatase (AP) - This is a 97% presumptive test for the enzyme PH level found primarily in semen.  This test will indicate the mass of the semen if the PSA test is positive.  Why the AP test should never be used alone (such as a home AP kit).
  • Human Salivary Amylase (HSA) - This is the first confirmatory test for human saliva. The outcome of this test will determine if oral sex may have been performed even if the semen detection is negative. (additional HSA information)
  • Human blood detection included on all male underwear (excluded when male underwear is obviously worn by female).
  • Sperm Protein (SP10) test is run when semen is detected above a calculated level.
  • Additional services

Time period of testing:

  • Testing is normally completed within 24 hours (Monday through Friday). Ninety-eight percent of all samples received before noon have been tested on the same day.  
  • Expedited same day service is available at an additional charge.

 - all at one low price of $199.00!  Please select this link to proceed to the order page.  Private Investigators please go to this link.

We guarantee you the most experienced laboratory semen detection test completed in the shortest period of time at the lowest cost or it's free.  See details.

Methods of Payment

Payment Accepted by the following:

(click logo for details)

Special instructions for payment made by
Money Order or Cashiers Check, Pay on Delivery

You should resolve your issues by purchasing from the absolute leader in semen detections... where your questions are answered with a conclusive 100% accurate report.

Additional Paid Services By Request

  • Guaranteed same day service.  Your test will take priority over all tests in process.
  • Multiple photos taken throughout test process
    -     $25.00 per article.  One photo is included with every order
  • Overnight return of sample and/or report
    -     Quoted once shipping address is provided and weight of package is determined.
  • DNA extraction and comparison analysis to prove that you are/are not the contributor to the stain



Semen and Sperm Detection, Incorporated (SSDI)
110 East Broward Blvd. Suite 1700
Fort Lauderdale, Florida 33301
517-336-7734 - 888-875-7574


Your test results are guaranteed to be confidential!